diff --git a/libp2p/host/basic_host.py b/libp2p/host/basic_host.py
index 24014321..60b31fe0 100644
--- a/libp2p/host/basic_host.py
+++ b/libp2p/host/basic_host.py
@@ -195,6 +195,29 @@ class BasicHost(IHost):
         net_stream.set_protocol(selected_protocol)
         return net_stream
 
+    async def send_command(self, peer_id: ID, command: str) -> list[str]:
+        """
+        Send a multistream-select command to the specified peer and return
+        the response.
+
+        :param peer_id: peer_id that host is connecting
+        :param command: supported multistream-select command (e.g., "ls)
+        :raise StreamFailure: If the stream cannot be opened or negotiation fails
+        :return: list of strings representing the response from peer.
+        """
+        new_stream = await self._network.new_stream(peer_id)
+
+        try:
+            response = await self.multiselect_client.query_multistream_command(
+                MultiselectCommunicator(new_stream), command
+            )
+        except MultiselectClientError as error:
+            logger.debug("fail to open a stream to peer %s, error=%s", peer_id, error)
+            await new_stream.reset()
+            raise StreamFailure(f"failed to open a stream to peer {peer_id}") from error
+
+        return response
+
     async def connect(self, peer_info: PeerInfo) -> None:
         """
         Ensure there is a connection between this host and the peer
diff --git a/libp2p/protocol_muxer/multiselect.py b/libp2p/protocol_muxer/multiselect.py
index ed9bccca..b7ee2004 100644
--- a/libp2p/protocol_muxer/multiselect.py
+++ b/libp2p/protocol_muxer/multiselect.py
@@ -60,8 +60,14 @@ class Multiselect(IMultiselectMuxer):
                 raise MultiselectError() from error
 
             if command == "ls":
-                # TODO: handle ls command
-                pass
+                supported_protocols = list(self.handlers.keys())
+                response = "\n".join(supported_protocols) + "\n"
+
+                try:
+                    await communicator.write(response)
+                except MultiselectCommunicatorError as error:
+                    raise MultiselectError() from error
+
             else:
                 protocol = TProtocol(command)
                 if protocol in self.handlers:
diff --git a/libp2p/protocol_muxer/multiselect_client.py b/libp2p/protocol_muxer/multiselect_client.py
index abbab54c..8d8c02a1 100644
--- a/libp2p/protocol_muxer/multiselect_client.py
+++ b/libp2p/protocol_muxer/multiselect_client.py
@@ -70,6 +70,36 @@ class MultiselectClient(IMultiselectClient):
 
         raise MultiselectClientError("protocols not supported")
 
+    async def query_multistream_command(
+        self, communicator: IMultiselectCommunicator, command: str
+    ) -> list[str]:
+        """
+        Send a multistream-select command over the given communicator and return
+        parsed response.
+
+        :param communicator: communicator to use to communicate with counterparty
+        :param command: supported multistream-select command(e.g., ls)
+        :raise MultiselectClientError: If the communicator fails to process data.
+        :return: list of strings representing the response from peer.
+        """
+        await self.handshake(communicator)
+
+        if command == "ls":
+            try:
+                await communicator.write("ls")
+            except MultiselectCommunicatorError as error:
+                raise MultiselectClientError() from error
+        else:
+            raise ValueError("Command not supported")
+
+        try:
+            response = await communicator.read()
+            response_list = response.strip().splitlines()
+        except MultiselectCommunicatorError as error:
+            raise MultiselectClientError() from error
+
+        return response_list
+
     async def try_select(
         self, communicator: IMultiselectCommunicator, protocol: TProtocol
     ) -> TProtocol:
diff --git a/newsfragments/622.feature.rst b/newsfragments/622.feature.rst
new file mode 100644
index 00000000..c9a83bb5
--- /dev/null
+++ b/newsfragments/622.feature.rst
@@ -0,0 +1,2 @@
+Feature: Support for sending `ls` command over `multistream-select` to list supported protocols from remote peer.
+This allows inspecting which protocol handlers a peer supports at runtime.
diff --git a/tests/core/protocol_muxer/test_protocol_muxer.py b/tests/core/protocol_muxer/test_protocol_muxer.py
index 151dfd07..98f48533 100644
--- a/tests/core/protocol_muxer/test_protocol_muxer.py
+++ b/tests/core/protocol_muxer/test_protocol_muxer.py
@@ -116,3 +116,35 @@ async def test_multiple_protocol_fails(security_protocol):
         await perform_simple_test(
             "", protocols_for_client, protocols_for_listener, security_protocol
         )
+
+
+@pytest.mark.trio
+async def test_multistream_command(security_protocol):
+    supported_protocols = [PROTOCOL_ECHO, PROTOCOL_FOO, PROTOCOL_POTATO, PROTOCOL_ROCK]
+
+    async with HostFactory.create_batch_and_listen(
+        2, security_protocol=security_protocol
+    ) as hosts:
+        listener, dialer = hosts[1], hosts[0]
+
+        for protocol in supported_protocols:
+            listener.set_stream_handler(
+                protocol, create_echo_stream_handler(ACK_PREFIX)
+            )
+
+        # Ensure dialer knows how to reach the listener
+        dialer.get_peerstore().add_addrs(listener.get_id(), listener.get_addrs(), 10)
+
+        # Dialer asks peer to list the supported protocols using `ls`
+        response = await dialer.send_command(listener.get_id(), "ls")
+
+        # We expect all supported protocols to show up
+        for protocol in supported_protocols:
+            assert protocol in response
+
+        assert "/does/not/exist" not in response
+        assert "/foo/bar/1.2.3" not in response
+
+        # Dialer asks for unspoorted command
+        with pytest.raises(ValueError, match="Command not supported"):
+            await dialer.send_command(listener.get_id(), "random")